![]() ![]() ![]() the site-specific recombination (region of crossover) in reverse orientation. The 34bp minimal FRT site sequence has the sequenceĥ' GAAGTTCCTATTC tctagaaaG tATAGGAACTTC 3'įor which flippase (Flp) binds to both 13-bp 5'-GAAGTTCCTATTC-3' arms flanking the 8 bp spacer, i.e. ![]() It is analogous to Cre- lox recombination but involves the recombination of sequences between short flippase recognition target ( FRT) sites by the recombinase flippase ( Flp) derived from the 2 µ plasmid of baker's yeast Saccharomyces cerevisiae. In genetics, Flp- FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism's DNA under controlled conditions in vivo. ![]()
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